This section provides an overview for counting chambers as well as their applications and principles. Also, please take a look at the list of 9 counting chamber manufacturers and their company rankings. Here are the top-ranked counting chamber companies as of February, 2024: 1.Structure Probe, Inc., 2.Hausser Scientific, 3.Abcam plc.
Figure 1. Overview of the blood cell calculator
A counting chamber is a tool used to count cells. It is also called a hemocytometer.
It is used for this purpose because it is necessary to count cells prior to cell fusion techniques or cell culture operations.
The size of cells varies depending on the object to be measured, such as blood cells (red blood cells, white blood cells, etc.), lymphocytes, or other cultured cells. For this reason, the scale width of the counting chamber is also different depending on the object to be measured.
The counting chambers are used in research and development of cell-related technologies. By calculating cell counts, it is possible to calculate cell density, survival rates, and other necessary values. This is always done as a preliminary preparation for cell culture operations such as transfection, cell fusion technology, cryopreservation, etc.
The counting chambers have series of equally spaced tick marks of different widths, each of which can be used to calculate a numerical value. The concentration of the suspension is adjusted so that there are about 100 cells within a frame of 1 mm per side, and the number of viable cells within a frame consisting of 16 squares is counted.
Figure 2. Image of how a cell looks
First, the cells to be observed are collected and resuspended in culture medium, from which the cell suspension is collected and colored with a dye to classify live and dead cells. Dead cells are colored, but living cells are not, so the presence or absence of color is used to distinguish between living and dead cells.
It is important to adjust the concentration of the suspension to an appropriate level so that there are about 100 cells in the frame because if the concentration is too high, it is difficult to measure, and if it is too low, it cannot be measured correctly. Initially, the number of viable cells within a 1 mm square frame, the largest of which consists of 16 squares, is counted.
By measuring the number of live and dead cells through this operation, the cell viability can be calculated.
The counting chambers are used with a cover glass adhered to it. The cover glass should be washed with ethanol and air-dried before use. Just before use, moisten the cover glass lightly with water and place it on the scale surface of the counting chambers. While being careful not to break the cover glass, apply light pressure as if rubbing the cover glass with your fingers to make it adhere closely to the counting chambers.
At this point, check for the presence of Newton rings to confirm that the glass is firmly adhered. Newton rings are rainbow-colored interference fringes that occur when glasses with the same percentage curve on their surfaces are superimposed on each other. They are not visible if dust or other contaminants have penetrated between the counting chambers and the cover glass.
If the Newton rings cannot be confirmed, wipe the surface of the counting chambers and the cover glass with a gauze or similar cloth moistened with an appropriate amount of ethanol, and repeat the same procedure. Note that the depth of the calculation chamber will change with dirt in it, and accurate counting will not be possible.
Some disposable products do not require a cover glass. In such cases, the labor of Newton ring creation can be eliminated.
Figure 3. Various types of scales
There are several types of counting chambers, each with a different scale inscription and depth of calculation chamber. The most commonly used type is the Bürker-Türk type counting chambers.
It is a duplex calculator with two calculation chambers and can count a wide range of cells, including not only red and white blood cells but also sperm, bacteria, yeast, plankton, and cultured cells.
In the Bürker-Türk type counting chambers, the volume of each calculation chamber is 0.9 μL, and the scale lines are designed so that each side of the scale is 3 mm in both length and width.
This single side is further trisected into nine 1 mm x 1 mm blocks. The middle block is mainly used for counting cells with large absolute numbers, such as red blood cells, while the four corner blocks are used for counting cells with relatively small absolute numbers, such as white blood cells and cultured cells.
Other types of counting chambers include the Thomas type, which is a single-chamber, single-stage calculator, and the modified Neubauer type, which is a double-stage calculator similar to the Bürker-Türk type but with simpler graduation lines.
*Including some distributors, etc.
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PerkinElmer Inc., founded in 1937, is an American manufacturer headquartered in Waltham, Massachusetts, specializing in laboratory technology and management. The company's product offerings include analysis and characterization instruments, software, and consumables. Such offerings cover chromatography, mass spectrometry, molecular and atomic spectroscopy. They also provide immunoassay and food quality analyzers, as well as the simultaneous thermal analyzer (STA). These products find applications in various industries, such as biopharmaceuticals, food safety, lithium battery, and water resource management. PerkinElmer also offers OneSource Laboratory Services, providing knowledge, application, and service solutions for scientific laboratories. Additionally, their LABStartUp program supports new laboratories with instrumentation, consumables, and funding.
Nexcelom Bioscience LLC, established in 2003 and based in Lawrence, Massachusetts, United States is a manufacturer of image cytometry products for cell analysis in life science and biometric research. The company produces different cell counters, such as brightfield automated counters, yeast automated cell counters, fluorescent automated cell counters, and high-throughput cell counters. The cellometer automatic cell counting products are used in cancer research and drug discovery. The machines are used in counting cells as small as 2 microns, such as human platelets, algae, and yeasts, and are acatered to biotechnology and pharmaceutical industries.
Hausser Scientific was established in 1989 in Horsham, PA, and is a manufacturer and supplier of glass counting chambers for counting cells and examining fluids and micro-organisms in laboratories. The company makes hemacytometers, bright-line mold counters, cover glasses, and pipettes delivered to national and international markets. Some of the products like Reichert bright line phase hemacytometer, Nageotte bright line chamber, Levy dark line hemacytometer, and Hausser bright line hemacytometer received a certification of counting chambers. The company also offers fabrication services like drilling, polishing, glass beading, and machining.
New York Microscope Company Inc. is based in Hicksville, New York, and is a full-service microscope company. The company serves government, medical, and industrial institutions including the USDA, NASA, Honeywell, and New York University. The company offers 3 varieties of microscopes: compound, stereo, and digital from brands such as Carson, Hitachi, Nikon, Pelican, and others. The company also offers services for repairing, reconditioning, and restoring microscopes. The company can perform calibration tests and fix alignment issues with microscopes.
Azer Scientific Inc. is a supplier of laboratory consumables and equipment. It is headquartered in Morgantown, Pennsylvania, and was established in 2003. The company supplies a wide range of clinical and life sciences laboratory consumables, including pipette tips, gloves, and lab coats. It also supplies multiple laboratory equipment, including centrifuges, microscopes, and pH meters. Azer Scientific also offers services such as custom labeling and packaging. Its products are used by many organizations, including pharmaceutical companies, biotechnology companies, and academic institutions.
Abcam Plc is a British producer and supplier of life science research materials that was established in 1998. Based in Cambridgeshire, United Kingdom, the company offers various cell and tissue products including stem cells and growth factors, proteins such as lysates and peptides, and primary as well as secondary antibodies (e.g. flow cytometry). It also provides various kits for measuring different aspects of living organisms, such as metabolism, immunology, and biomarkers. Abcam Plc serves clients in a wide range of biomedical operations, including pharmaceutical production, vaccine development, and institutional academic research.
Buch & Holm, based in Denmark was founded in 1935, is a supplier of equipment for laboratories and industrial companies representing a large number of the world's manufacturers with specialties in each of their fields. The company offers a complete service package with customer support and technical service, webshop etc. They supply products and solutions to various research, healthcare, and educational institutions. Their comprehensive product range includes laboratory instruments, consumables, and chemicals, catering to different scientific needs.
Structure Probe Inc, founded in the year 1970, based in Pennsylvania, United States is a manufacturer and supplier of high quality silicon AFM probes. Their product portfolio includes tweezers, small tools, vacuum pumps and tools, SEM mounts, instruments, TEM grids, chemicals, cleaning supplies, microscopic calibration, specimen storage, substrates, filtration, maintenance, nanomaterials, paint, and adhesives. The products are used in multiple applications which include SEM and TEM supplies, SPM and APM supplies, light microscopic related products, material science, and life science sample preparations.
Electron Microscopy Sciences, established in 1974, is a supplier of scientific products and services, based in the United States. The company specializes in providing instruments, consumables, and chemicals for electron microscopy and histology, and they are the most comprehensive source for all fields of microscopy, histology and general laboratory research. Their network spans over 50 countries, catering to research institutions, universities, and industries worldwide. Notable achievements include strong partnerships with major manufacturers, ensuring top-notch offerings for customers.
*Including some distributors, etc.
*Including some distributors, etc.
|Number of Companies
|United States of America
5 products are listed.