This section provides an overview for elisa as well as their applications and principles. Also, please take a look at the list of 20 elisa manufacturers and their company rankings. Here are the top-ranked elisa companies as of November, 2024: 1.FIRICH ENTERPRISES CO, LTD., 2.LifeSpan Bio Sciences, Inc., 3.biolegend.
Table of Contents
ELISA is a method for qualitative and quantitative analysis of proteins, such as antigens and antibodies present in liquid solutions. ELISA is an abbreviation for enzyme-linked immuno sorbent assay and is a type of immunological assay. ELISA utilizes the specificity of antigens and antibodies and is a highly accurate method for both qualitative and quantitative analysis.
ELISA is widely used for quantitative analysis of antigens and antibodies, as commercial kits are available. In general, temperature control is important for ELISA kits, so care should be taken to ensure that the kits are not kept at extremely different temperatures depending on the location of the refrigerator where they are stored, or that they are not frozen due to excessive cold.
Depending on the method, ELISA may require many steps before quantitative analysis, but recently, fully automated analyzers have been developed, and the time required for analysis has been reduced.
ELISA is widely used in the field of biotechnology. For example, ELISA is used for analyzing antigens and antibodies in blood, such as plasma and serum. By collecting blood samples and measuring the values of autoantibodies in ELISA, it is possible to diagnose diseases or to determine antibody titers due to vaccination. For this reason, ELISA is widely used in the analysis of blood tests performed in hospitals, and even recently, more and more diseases have been identified by ELISA through research.
It can also be used to determine the presence or absence of allergens in food, or to determine the presence or absence of food allergies by collecting blood samples from the subject.
While the PCR method using DNA has become well known as a test for coronavirus antigens and antibodies, ELISA analysis is also available in kits and other forms.
ELISA uses an antigen-antibody reaction to perform the analysis.
First, the antigen to be measured is combined with an antibody that has specificity to the antigen. To detect the bound antibody, an antibody labeled with a specific enzyme is further bound.
The antigen, antibody, and enzyme-bound substance to be measured are then mixed with a substrate that reacts with the enzyme to produce a color change. The color tone changes in accordance with the amount of the substance to be measured, and the absorption spectrum is measured using an absorbance spectrophotometer for quantitative and qualitative analysis.
IgG antibodies are often used in immunological assay methods, including ELISA.
There are several methods of ELISA, and many types of kits are available. The simplest direct method is introduced here. The basic flow of measurement is not much different from others.
First, an antibody that binds to the substance to be measured is adsorbed on a microplate.
A defined amount of liquid sample is added to the microplate using a pipette. The temperature is controlled in an incubator, and the sample is allowed to react and bind to the antigens and antibodies on the microplate. Excess antibodies are washed away.
The bound antibodies are further reacted by specific enzymes. To detect enzyme activity, a substrate with a different absorption spectrum when bound to the enzyme is added, and the absorption spectrum after the reaction is measured and analyzed. In many cases, peroxidase (HRP) derived from horseradish is used as a substrate for color development, and care must be taken to avoid using blood collection tubes coated with sodium azide or other inhibitors when using HRP.
Finally, the absorption spectrum is measured and analyzed using an absorbance spectrophotometer.
ELISA often requires many steps for accurate analysis depending on the substance to be measured, and each step is repeated, such as aliquoting and washing. Pipettors, microplates, incubators, absorbance analyzers, etc. are also required.
Recently, devices have been developed that enable a single unit to perform all roles, from pipetting to absorbance analysis. The ability to perform measurements fully automatically, without human intervention, reduces errors and saves time and labor.
*Including some distributors, etc.
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Custom Industrial Products, founded in 1986 and based in Melbourne, FL, is a manufacturer of vertical lifts. The company also provides industrial material baskets, various lines of safety products, and more. With one of the biggest powder coating operations in Central Florida, the firm makes use of an automated conveyor powder coating system. The team ensured the lift could support 5,000 pounds and ran at the desired 25 feet per minute. The company is specialised in producing modular VRC lifts,custom VRC lifts, material handling baskets, and safety products.
Boster Biological Technology Co., Ltd., founded in 1993 and based in Pleasanton, California, USA, is a manufacturer of life sciences equipment and products. Specializing in the development and production of advanced research reagents, the company offers a broad range of products, including ELISA Kits and antibodies, to support scientific research and diagnostics. It holds ISO 13485 certification, for the consistency of its products and the products find applications in various industries, such as academic research, pharmaceutical development, and clinical diagnostics.
BioLegend is a manufacturer and supplier of antibodies, proteins, and reagents for life science research that was established in 2002 and headquartered in San Diego, California. The company produces multiple types of antibodies, proteins, and reagents, including primary antibodies, secondary antibodies, and cell culture media. Its products such as antibodies and reagents, apoptosis and cell health reagents, buffers and chemical probes, specific reagents, isotype controls, and magnetic cell separation - mojosort(trademarked) are used by a number of researchers in academia, pharmaceutical, and biotechnology industries. The company also provides a range of services, including custom antibody development, assay development, and training.
LifeSpan BioSciences, Inc. is a manufacturer of antibody reagents founded in 1995 and headquartered in Massachusetts, USA. The company specializes in antibody production, validation, and immunohistochemistry. Its reagent catalog contains over 750,000 products, including monoclonal and polyclonal antibodies, immunoassays and kits, recombinant proteins, biochemicals, and cell lysates. The company maintains a global network of distributors, spanning across Sweden, Norway, Denmark, and Finland. It is a part of Absolute Biotech and serves the life sciences sector.
Bio-Techne, established in 1970 and headquartered in Minneapolis, USA, is a supplier and manufacturer of essential tools for life science research, therapeutics, and clinical diagnostics. The company offers an extensive product range, including purified proteins and reagent solutions, with a notable emphasis on cytokines and growth factors, along with antibodies, immunoassays, biologically active small molecule compounds, tissue culture reagents, and gene therapy workflow solutions, including T-Cell activation technologies. Furthermore, it provides protein analysis solutions, streamlining researchers' workflows for automated Western blot and multiplexed ELISA techniques.
Biomatik Corporation, founded in 2002 and headquartered in Cambridge, Ontario, Canada, is a manufacturer of biotechnology products and integrated life sciences. The company specializes in the production of BioReagents, ELISA kits, & Peptide Synthesis, and Protein & Antibody Services, serving the comprehensive needs of the global research community. It holds ISO 9001 certification, and scientists utilize its extensive product range, facilitating advancements in genomics, proteomics, and immunology research. The adaptable applications of its offerings span academic research, pharmaceutical development, and clinical diagnostics.
Firich Enterprises Co. Ltd, founded in 1995 and located in FEC Netherlands, is a manufacturer of POS systems, Mobile POS, FEC platforms, BOX systems, and FEC systems. The company’s products are monitors, printers and nubis, visio objects, and peripherals. The products are used in businesses to make payments through QR codes and facilitate store lines to keep the business flowing. The company has passed the quality and environmental management systems certificates.
Millipore Sigma, formerly known as Sigma-Aldrich, was created in 175 through the merger of Sigma Chemical Company and Aldrich Chemical company and today is a subsidiary of Merck. Millipore Sigma is a life science and biotechnology company serving the industry with several products and services. Some of their product categories include peptide synthesis materials, biochemicals, building blocks, catalysts, chemical biology, chemical synthesis, flavors & fragrances, formulation, lab chemicals, lab safety, APIs, and stable isotopes. Services include characterization, contract manufacturing, custom products, software, support, testing, and mRNA development & manufacturing.
Alfa Aesar is based in Lancashire, United Kingdom. Alfa Aesar is part of Thermo Fisher Scientific, is a manufacturer and supplier of chemicals, metals, and life science products for research and development. Alfa Aesar’s product lines include chemicals, life science, materials & metals, catalysts, and analytical & lab ware. Chemical offerings include inorganic compounds, organic compounds, organometallic, and solvents. Life sciences include biotechnology products, cellular & molecular biology, genomics, and immunology. Materials & metal include alloys, carbon, etchants, and more. Catalysts include ferrocenes, fuel cell products, and precious metals & catalysts. Alfa Aesar also offers custom manufacturing options.
Cell Signaling Technology, established in 1999 and headquartered in Danvers, Massachusetts, is a life sciences company developing products for other researchers. The company offers nature biotechnology, cell, journal of immunology, and biological chemistry research. It is effective in applied systems biology research, particularly as it relates to cancer, and understands the importance of using antibodies. All of its antibodies undergo rigorous validation for multiple applications to ensure efficiency in research. It also provides technical support, methodologies, and guidance for solid research outcomes.
Abbexa, founded in the UK in 2012, is a supplier of biological tools for the life science, pharmaceutical development, and biotechnology sectors. The company offers a wide variety of laboratory tools, including primary antibodies, secondary antibodies, and proteins. The company has 2 branches in the UK, including a state-of-the-art testing and research lab, a location in Houston, Texas, USA, and a location in Leiden, The Netherlands, to serve customers worldwide.
Ethos Biosciences, founded in the United States in 1988, is a supplier of diabetes-related diagnostic products. The company offers blood glucose monitoring systems that are accurate, easy to use, and designed to help individuals keep their glucose levels in check and range of insulin delivery solutions includes insulin pumps and pens. It is a family owned and operated group of specialty chemical manufacturing companies with expertise in monomers, polymers, microspheres, and reagents for the pharmaceutical, medical device, and diagnostic industries.
Ranking as of November 2024
Derivation MethodRank | Company | Click Share |
---|---|---|
1 | FIRICH ENTERPRISES CO, LTD. |
11.1%
|
2 | LifeSpan Bio Sciences, Inc. |
7.2%
|
3 | biolegend |
7.2%
|
4 | Custom Industrial Products |
6.9%
|
5 | Bio-Techne |
6.8%
|
6 | Boster Biological Technology Co Ltd |
6.7%
|
7 | Gold Standard Diagnostics Horsham Inc. |
6.2%
|
8 | EUROGENTEC |
5.4%
|
9 | Sino Biological, Inc. |
5.4%
|
10 | Eagle Biosciences |
5.2%
|
Derivation Method
The ranking is calculated based on the click share within the elisa page as of November 2024. Click share is defined as the total number of clicks for all companies during the period divided by the number of clicks for each company.Number of Employees
Newly Established Company
Company with a History
*Including some distributors, etc.
*Including some distributors, etc.
Country | Number of Companies | Share (%) |
---|---|---|
United States of America | 8 | 57.1% |
United Kingdom | 3 | 21.4% |
Canada | 1 | 7.1% |
Taiwan | 1 | 7.1% |
Japan | 1 | 7.1% |