This section provides overview, applications, and principles of pipette tips. Also, please take a look at the list of 14 pipette tip manufacturers and their company rankings.
A pipette tip is a plastic conical tube attached to a micropipette. Pipette tips are used for measuring solutions in the order of μL to mL in research, development, and quality control in the chemical, biological, and other fields. This operation of measuring solutions by aspiration and ejection of a pipette is called pipetting.
Different sizes of pipettes are available for different sizes of pipettes, and the color of the pipette may vary depending on the size. To prevent contamination, pipettes should be stored in dedicated racks and discarded once used.
Pipette tips are attached to micropipettes and used in all fields related to chemistry and biology. Specific uses include laboratory experiments in the field of biochemistry, quality control at pharmaceutical manufacturers, and specimen collection in clinical testing. Micropipettes are used for handling small amounts of liquids ranging from µL to several mL.
Although it is possible to quickly collect a fixed amount of liquid by simply pressing a button, it requires more care in operation than a glass volumetric analyzer because the volume tends to change more easily than a glass volumetric analyzer.
Pipette tips are filled into a special rack for pipette tips and attached by directly inserting the tip of a micropipette. Aspirate liquid by slowly pushing and releasing the micropipette push button to the first stage, and when dispensing, push the button to the second stage for full dispensing. To dispose of the pipette tip after use, simply push the ejector button. Since there is no need to touch the pipette tip by hand, contamination of the sample and adhesion of chemicals to the operator can be prevented.
There are various types of pipette tips. The capacities of pipette tips mainly used are 10 µL, 200 µL, and 1000 µL. Other types include 250 µL and 30 µL, and larger 5 mL and 10 mL pipette tips.
Since pipette tips come in several sizes, it is important to select the appropriate size micropipette and pipette tip according to the volume of liquid to be weighed. Specifically, the maximum volume that can be weighed by the micropipette should be close to the volume of the liquid to be weighed. This is important to minimize the error of the liquid volume.
For accurate measurement with pipette tips, the following points should be noted.
Due to the material of pipette tips, solution components may be adsorbed to the inner wall of the pipette tip. It is effective to aspirate and dispense the liquid to be weighed at least twice and pre-rinse the pipette before use.
The angle of entry of the tip should be as close to 90 degrees to the liquid surface as possible. It is appropriate to maintain within 20 degrees of vertical. If the pipette is held at an angle close to horizontal, an excessive amount of liquid may be aspirated. 3.
It is known that if the tip is immersed too deeply, the gas inside the tip is compressed and too much liquid is aspirated. It is appropriate to immerse the tip at a depth of 1 to 2 mm for micro volume pipettes and at a maximum of 3 to 6 mm for normal volume pipettes.
First of all, when aspirating liquid, care should be taken to raise the finger slowly, without abruptly releasing it from the button. The purpose is to minimize the difference in aspiration speed due to the difference in viscosity of the liquid, and to prevent the pipette from being broken when the rapidly aspirated liquid enters the mechanical part of the pipettor. When dispensing, even the last remaining droplet should be dispensed completely, so that it does not adhere to the tip of the tip. In particular, it is recommended that the tip tip be dispensed along the wall of the container.
When weighing liquids with low surface tension, such as organic solvents, it should be noted that the liquid may remain adhered to the outer wall. In some cases, it may be more appropriate to use a microneedle with a needle-like tip.
*Including some distributors, etc.
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