Specific Single-Cell Western (Single Cell Western)
Protein Simple Japan
About This Product
Milo ™ (Myro) can identify the subpuration of cells that cannot be seen in conventional Western blots. In other words, it is possible to study the unique cell type in uneven trials. Is there a protein target that can not be measured with flow site metreal? Please try Myro. Using antibodies verified for commercially available western blots, you can measure targets without intracellular proteins, transcription factors, phosphorylated proteins, or without flows. Have you already studied single cell RNA? Myro can directly verify the protein targeted by RNA targets of single cells, so the result of gene expression is more reliable.
MILO Milo can get a single cell western result in about 4 hours using Scwest ™ chips. When the cell suspension is loaded, the scwest chip distributes cells and captures 1000 individual cells. Milo then performs high-speed SDS-PAGE for one minute on each single cell license on the on-chip. And on the chips, you can apply a conventional Western antibody to survive up to 4 targets per cell at the same time.
･ Eating subpopulation and protein isoform
MILO Milo measures protein expression in 1000 individual cells. It means that the cells of the sample can identify which cells represent each protein. Using that information, you can identify cell subpurations that cannot be measured in the conventional (population) Western blot. In addition, Milo can also split protein isoforms that cannot be distinguished by flow site metreal. Myro provides information about your cells that cannot be obtained by other methods.
･ Exit diversity of protein
You can easily find out which cell subtype is contributing to your bulk sample. If all cells are evenly expressed in the target, or if you are wondering if you are involved in some high -expression and low -priced current, Myro will help.
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Product
Specific Single-Cell Western (Single Cell Western)
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Handling Company
Protein Simple Japan
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Categories
1 Models of Specific Single-Cell Western (Single Cell Western)
| Product Image | Part Number | Price (excluding tax) | Cell diameter | Corresponding cells | Electric swimming distance | Molecular weight range | Molecular weight resolution | Necessary device | Number of wells on ScWest chip | Sample type | Scwest chip size | Total workflow time | antibody | size | weight |
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Milo (Milo) |
Available upon quote | 7-25μm | Mammamic cells, spherical, unpolished in suspension | <0.9 m m | 15-175KDA | 10 % for specific wavelengths and 30 % or less for the same wavelength | Open format micro -race cannanner, resolution 10μm or more | 6,400 | > 10,000 cell suspensions | 1in.x3in. Glass slide | 4-6 hours | Primary antibodies, fluorescent label secondary antibodies | H15cmxw28cmxd33cm | 4.5kg |
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