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This section provides an overview for hplcs as well as their applications and principles. Also, please take a look at the list of 8 hplc manufacturers and their company rankings. Here are the top-ranked hplc companies as of January, 2025: 1.KNAUER Wissenschaftliche Geräte GmbH, 2.Chrom Tech, Inc, 3.Spectory.
Table of Contents
HPLC (High-Performance Liquid Chromatography) is a method for separating and detecting individual compounds in a sample through the interaction between a column and the sample. It is highly effective for measuring and detecting trace components and is widely used in industries such as pharmaceuticals, biochemistry, food, and environmental studies.
Since the peak area of HPLC is proportional to the sample concentration, it is possible to quantify the concentration of components in the sample. The separation behavior of samples in HPLC varies depending on the column and mobile phase, requiring the design of appropriate analysis conditions.
High Performance Liquid Chromatography (HPLCs) are analytical method that utilize the interaction between a column and a sample to separate each component within a sample. The method is straightforward: analysis can be performed by directly injecting the sample solution or by placing the sample solution in the autosampler for batch processing.
HPLCs are utilized in various industries. In the pharmaceutical field, they are used to analyze trace impurities and active ingredients. In the food, beverage, and environmental sectors, they analyze nutritional ingredients, functional components, additives, and pesticide residues. In biochemistry, HPLCs are employed for analyzing proteins and nucleic acid-related substances.
HPLCs and gas chromatography (GC) are types of chromatography. This method separates compounds contained in an analyte by passing them through a column, allowing adsorption to the column or other media. The medium through which the sample components flow, such as a solvent in HPLCs or gas in GC, is called the "mobile phase," while the medium to which the sample adsorbs, like a column, is the "stationary phase."
The type and strength of interaction between the sample components and the column vary depending on the column type. For example, in an ODS column, an alkyl chain (octadecyl group) is bonded to the column, enabling the sample's adsorption through hydrophobic interaction. Silica gel columns have silanol groups on the surface that adsorb polar compounds. Other column types include those modified with phenyl groups, cyano groups, and amino groups.
The peak area of HPLCs is proportional to the concentration of a sample. However, when using a UV detector, the peak area varies with the absorbance coefficient (ease of light absorption), even if the sample concentration is the same. Therefore, when analyzing concentrations using HPLCs, it is necessary to prepare standard samples for area comparison.
One concentration analysis method is the "external standard method". This involves preparing multiple standard samples with known concentrations for HPLC analysis and determining the peak areas. Since the concentration of each sample is known, an equation for determining concentration from the area value can be obtained by plotting the area value against the concentration.
The second method is the "internal standard method". Here, another compound, chemically and physically stable, is added as an internal standard to a standard sample with a known concentration. After addition, HPLC analysis is performed to determine the ratio of the standard sample's peak area to that of the internal standard. A calibration curve can be obtained using the ratio of the added internal standard amount as the abscissa and the peak area ratio as the ordinate.
A variety of instruments serve as HPLC detectors, including ultraviolet-visible (UV-Vis) detectors, fluorescence detectors, and differential refractive index detectors (RID). The detection limits of these detectors vary widely depending on the sample. For instance, the UV-Vis detector has a detection limit of approximately 10 picograms (pg), and the fluorescence detector is 0.1 pg.
The most sensitive is the mass spectrometer (MS), estimated to have a detection sensitivity of 0.01 pg. However, the detection limit depends on the type and concentration of compounds in the sample, the degree of separation, and sometimes derivatization. Optimization of HPLC analysis, including sample pretreatment, is necessary for high-sensitivity detection.
*Including some distributors, etc.
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Thermo Fisher Scientific Inc., founded in 1956 and headquartered in Waltham, Massachusetts, is a manufacturer and supplier of life science solutions, analytical instruments, specialty diagnostics, laboratory products, and biopharma services. Through its brand names, including Invitrogen, Fisher Scientific, Patheon, Applied Biosystems, and Gibco, among others, the company provides a wide range of products, which include chromatography systems, thermal cyclers, automated cell counters, fermenters, and DNA polymerases. The company’s annual revenue is over 40 billion USD, and it serves several fields, including customers working in clinical diagnostic labs, research institutions, hospitals, government agencies, and pharmaceutical/biotech companies.
Chromtech is a company that specializes in the distribution of chromatography products, laboratory supplies, and scientific equipment. They offer a wide range of products including gas chromatography systems, liquid chromatography systems, columns, sample preparation products, and more. Chromtech aims to provide their customers with high-quality products, exceptional service, and technical support to help them achieve their goals. Their website provides customers with detailed information on their products and services, and offers easy online ordering.
SHIMADZU established in Duisburg, Nordrhein-Westfalen, in 1968 is a manufacturer of Analytical and Measuring Instruments and medical systems. Their product portfolio includes analytical instruments, including spectrophotometers, chromatography systems, mass spectrometers, and elemental analyzers, used for precise chemical analysis and research. The company serves industries such as Pharmaceutical and Biotechnology, Research and Development and Chemical and Petrochemical. The company has a global distribution channel and the company also offers customer services that include support, custom research projects and customized services.
Merck, started in 1668 and based in Darmstadt, Germany, is a supplier of solutions and services for research, development, and manufacture of pharmaceutical and biotechnological drug therapies. The company serves the life science industry and has a broad portfolio of 300,000 products, including biopharmaceutical manufacturing, industrial microbiology, reagent, life science research, and water purification products. It is available in 66 countries, brings together six brands, and has several ISO certifications, including ISO 13485, ISO 14001, ISO 13485, and ISO 50001.
Waters Corporation is an ISO 9001-certified manufacturer of laboratory consumables, equipment, and software established in 1958 in Milford, Massachusetts, USA. The company’s products include mass spectrometers for measuring mass-to-charge ratios of ions, liquid chromatography for separating or quantifying the components of different mixtures, and data analytics software for processing and managing data on a cloud-enabled platform. It also offers consumables and supplies for maintaining or enhancing the reliability of its portfolio products. The company’s products are used mainly by its clients in the biopharma, materials science, and biomedical research sectors.
KNAUER, established in 1962, manufactures high-end scientific instruments for research, routine analysis, quality assurance, and other applications based in Berlin, Germany. The company has supported technologies including liquid chromatography, precise handling and pumping of liquids up to high pressures, as well as flow-through detection of dissolved substances. Customizable products and solutions are one of its strengths, and it produces high-tech laboratory instruments for research, routine analysis and production. The product range includes systems, engineering solutions and components. Today, it is an established company with 180 employees that successfully develops, manufactures and markets chromatography instruments worldwide.
Ranking as of January 2025
Derivation Method| Rank | Company | Click Share |
|---|---|---|
| 1 | KNAUER Wissenschaftliche Geräte GmbH |
22.6%
|
| 2 | Chrom Tech, Inc |
18.4%
|
| 3 | Spectory |
12.0%
|
| 4 | Merck |
12.0%
|
| 5 | Thermo Fisher Scientific Inc. |
10.8%
|
| 6 | Waters Corporation |
8.9%
|
| 7 | Shimadzu Seisakusho Co., Ltd. |
7.8%
|
| 8 | SHIMADZU |
7.5%
|
Derivation Method
The ranking is calculated based on the click share within the hplc page as of January 2025. Click share is defined as the total number of clicks for all companies during the period divided by the number of clicks for each company.Number of Employees
Newly Established Company
Company with a History
*Including some distributors, etc.
*Including some distributors, etc.
| Country | Number of Companies | Share (%) |
|---|---|---|
|
United States of America
|
2 | 40.0% |
|
Germany
|
2 | 40.0% |
Japan
|
1 | 20.0% |
47 products found
47 products
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INERTSUSTAIN AQ-C18 is an ODS column designed to keep high-polar compounds more strongly compared to general ODS columns while having high inactivi...
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