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Bioassay Products OsteoImage™ Mineralization Assay
Lonza Co., Ltd.

Lonza Co., Ltd.'s Response Status

Response Rate

100.0%

Response Time

168.1hours


About This Product

The OsteoImage™ Mineralization Assay is a rapid, fluorescent, in vitro assay for assessing bone cell mineralization. This assay allows the quantification of in vitro mineralization by osteoblastic stem cells, primary osteoblasts, and osteoblast-like cell lines. This assay detects the specific binding of fluorescent OsteoImage™ staining reagent to the hydroxyapatite moiety of deposited osteoid cell nodules. This assay is sensitive enough to detect time-dependent increases in mineralization in differentiating osteoblast culture media. Unlike common histochemical methods (such as von Kossa staining and Alizarin Red staining, which do not exhibit specificity for hydroxyapatite), the OsteoImage™ assay eliminates multiple steps and tedious extraction procedures. This latest addition to Lonza's bone research product line improves the speed, sensitivity, and ease of measuring mineralization in cell culture.

■Advantages

・Capable of quantitative plate measurements or qualitative and visual fluorescence microscopy ・Can be used with primary osteoblasts, osteoblastic stem cells, and osteoblast cell lines ・Measure hydroxyapatite in the same way as bone ・Complete within 90 minutes (no complicated extraction operations) Sufficient sensitivity to detect time-dependent increases in mineralization in differentiating cells ・Can be used from 6-well plates to 96-well plates

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    Bioassay Products OsteoImage™ Mineralization Assay

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1 Models of Bioassay Products OsteoImage™ Mineralization Assay

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OsteoImage™ Mineralization Assay

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Response Rate

100.0%


Response Time

168.1hrs

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