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  4. Bioassay Products ToxiLight™ Nondestructive Cytotoxicity Bioassay Kit (Lonza Co., Ltd.)

Bioassay Products ToxiLight™ Nondestructive Cytotoxicity Bioassay Kit
Lonza Co., Ltd.

About This Product

ToxiLight™ Non-destructive Cytotoxicity Bioassay Kit is a bioluminescent, non-destructive cytotoxicity assay kit that measures adenylate kinase (AK) released from damaged cells. AK is a stable protein present in all eukaryotic cells and is released into the culture medium upon cell death. Due to the activity of AK, ADP (adenosine diphosphate) is phosphorylated to generate ATP (adenosine triphosphate), and the generated ATP is measured using a luciferase reaction. As the level of cell lysis increases, the amount of AK in the medium supernatant also increases, resulting in the generation of high intensity light by the ToxiLight™ reagent. Cell lysis is not required and measurements are performed using the medium supernatant.

■Advantages

・High sensitivity – detects as few as 10 cells ・Non-destructive – No lysis required, allowing continuous analysis of the same sample ・Simple – just add a single reagent to cell culture medium or medium supernatant Rapid – results from 96-well plates can be analyzed in less than 10 minutes ・Applicability – Culture supernatant can be stored frozen without reducing AK activity

■Applications

・Cytotoxicity research ・High Contest Screening ・Combination assay

  • Product

    Bioassay Products ToxiLight™ Nondestructive Cytotoxicity Bioassay Kit

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About Company Handling This Product

Lonza Co., Ltd.

  • Japan

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