Microscope Laser Interface MicroPoint-MicroPoint
Microscope Laser Interface MicroPoint-Photonic Instruments Co., Ltd.

Microscope Laser Interface MicroPoint
Photonic Instruments Co., Ltd.


About This Product

Laser light is introduced from the epi-illumination device attachment point of the optical microscope and irradiates the sample under the objective lens. By focusing the light onto the sample using the focusing power of the objective lens, it is possible to ablate individual cells or optically stimulate microscopic areas easily, safely, accurately, and without contamination. Can be attached to most optical microscopes without modification. A standard single beam type and a galvano type with a built-in simple scanner that can irradiate the laser to any position within the field of view are available. ■Features ・Extremely small optical stimulation: a minute spot close to the diffraction limit (less than 0.3 μm when using a 100X objective) ・Laser and observation light source are coaxial: Laser irradiation can be performed at the same time as observation. ・Selective irradiation is possible in the depth direction of the sample - Easy to install and move due to fiber connection method. - Adopts dye laser, wavelength can be changed depending on purpose. (Selectable range 365nm to 900nm) ・Pulse energy 50μJ or more, pulse width 2-6 nsec. ・Beam alignment and power adjustment can be easily performed. ・Can be mounted on upright/inverted optical microscopes from Olympus, Nikon, Leica, and Zeiss application ■Laser Ablation It is possible to cut, destroy, or damage specific parts of a sample. Targets: Nematodes, flies, zebrafish, plant roots, yeast, etc. Examples: nerve severing, microorgan destruction, cell damage ■Photobleaching/Photobleaching Irradiating the fluorescent dye with strong light causes it to fade. We will use this phenomenon to conduct FLIP and FRAP experiments. ■Photoconversion/Photoconversion The fluorescence wavelength is shifted by irradiating it with light of a specific wavelength. ■Photoactivation/Photoactivation The fluorescence intensity is amplified by irradiating fluorescent proteins such as PA-GFP and PA-RFP with light of a specific wavelength. ■Photoswitching The fluorescence wavelength can be reversibly changed by irradiating it with light of a specific wavelength. You can also experiment with the phenomenon of photochromism using photochromic compounds under a microscope.

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    Microscope Laser Interface MicroPoint

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1 Models of Microscope Laser Interface MicroPoint

Product Image Part Number Price (excluding tax) Continuous shot stability accuracy Dimensions (W x H x D) Nitrogen gas supply method Peak output Pulse energy Pulse width Repetition rate Wavelength Weight
Microscope Laser Interface MicroPoint-Part Number-MicroPoint

MicroPoint

Available upon quote Cartridge type <± 3.5 %
Gas flow type <± 2.5 %
Cartridge type 95 x 95 x 279 mm
Gas flow type 300 x 150 x 580 mm
Cartridge type Cartridge replacement
Gas flow type gas equipment (cylinders and facility piping)
Cartridge type 45 kW
Gas flow type 330 kW
Cartridge type 150 μJ
Gas flow type 300 μJ
Cartridge type 4 ns
Gas flow type 0.9 ns (900 ps)
1-20Hz 337.1nm Cartridge type 3.4 kg
Gas flow type 13 kg

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