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  2. Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay (Lonza Co., Ltd.)
Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay-PPiLight™ Inorganic Pyrophosphate Assay
Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay-Lonza Co., Ltd.

Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay
Lonza Co., Ltd.

Lonza Co., Ltd.'s Response Status

Response Rate

100.0%

Response Time

88.9hours

About This Product

Inorganic pyrophosphate (PPi) is a diphosphate molecule that is used as a substrate or product in a wide variety of enzymatic reactions. Enzymes that use PPi as a substrate include phosphotransferases and pyrophosphatases. Enzymes that produce PPi are more numerous and include cyclases, hydrolases, and ligases. PPiLight™ Inorganic Pyrophosphate Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi, the detection reagent catalyzes the conversion of AMP to ATP. The assay uses luciferase and detects a luminescent reaction due to newly formed ATP and luciferin.

■Advantages

・Fast – Enzyme activity due to pyrophosphate consumption can be measured within 1 hour ・Simple – two-step luminescent assay (no radioactive substrate, beads, or antibodies required) ・Wide detection range – measurement range from 0.02 μM to 10 μM ・High sensitivity – Can measure up to 0.02 μM Versatility – available in 96, 384 and 1586 well formats

■Applications

・Measure phosphotransferase and pyrophosphatase activities ・Measures cyclase, ligase, hydrolase and DNA polymerase activities

  • Product

    Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay

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1 Models of Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay

Image Part Number Price (excluding tax) Number of inspections Operating temperature Reproducibility Assay time PPi range
Bioassay Products PPiLight™ Inorganic Pyrophosphate Assay-Part Number-PPiLight™ Inorganic Pyrophosphate Assay

PPiLight™ Inorganic Pyrophosphate Assay

Available upon quote

500 times (96 well or 386 well plate)

Normal temperature

R^2>0.95

1 hour

0.02 μM~10 μM (for 100 μl sample)

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About Company Handling This Product

Lonza Co., Ltd.

Response Rate

100.0%

Response Time

88.9hrs

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