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  2. Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay (Lonza Co., Ltd.)
Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay-PDELight™ HTS cAMP Phosphodiesterase Assay
Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay-Lonza Co., Ltd.

Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay
Lonza Co., Ltd.

About This Product

The PDELight™ HTS cAMP Phosphodiesterase Assay Kit is a versatile assay designed for high-throughput screening for the identification and IC50 determination of inhibitors of phosphodiesterase activity. This assay employs a stable and sensitive luciferase-based bioluminescent system to quantify AMP hydrolyzed from cAMP by phosphodiesterases. AMP is directly converted to ATP, and for each molecule of converted ATP, nearly one photon of light is emitted. The assay is sensitive, stable, and reproducible. Unlike other phosphodiesterase assays, the PDELight™ kit does not require radiation, beads, modified substrates, or antibodies.

■Advantages

・Easy – measurement results can be read by adding one type of reagent to the reaction ・Universal platform – can be used for all cAMP-dependent phosphodiesterases Rapid assay – complete assay in 384-well plate in less than 3 minutes Sensitivity – Enzymes can be used in 96-well, 384-well, or 1536-well formats Reproducible and stable assay – typical Z’ value >0.8, small error, excellent sensitivity

■Applications

・cAMP-dependent phosphodiesterase activity screening ・IC50 measurement

  • Product

    Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay

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1 Models of Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay

Image Part Number Price (excluding tax) Reproducibility Phosphodiesterase Assay time AMP range
Bioassay Products PDELight™ HTS cAMP Phosphodiesterase Assay-Part Number-PDELight™ HTS cAMP Phosphodiesterase Assay

PDELight™ HTS cAMP Phosphodiesterase Assay

Available upon quote

Typical coefficient of variation (CV) less than 5%, typical Z’ value greater than 0.8

CAMP phosphodiesterase

Less than 3 minutes per plate

<10 nM~20 μM

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About Company Handling This Product

Lonza Co., Ltd.

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