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  2. Gene introduction for primary adipocytes (Lonza Co., Ltd.)
Gene introduction for primary adipocytes-Nucleofector™ kit for human preadipocytes
Gene introduction for primary adipocytes-Lonza Co., Ltd.

Gene introduction for primary adipocytes
Lonza Co., Ltd.

About This Product

A new gene transfer technology designed to meet the needs of cell lines. This is a non-viral method based on a unique combination of electrical parameters and cell type-specific reagents. The best Nucleofection™ conditions are determined by the individual cell type, not the substrate being transfected. This means that the exact same conditions apply for Nucleofection™ of DNA and RNA. This makes substrate switching or simultaneous DNA and RNA transfection very easy. Nucleofector® technology is non-viral, but allows for the direct introduction of DNA into nucleic acids. This gives Nucleofection™ the ability to transduce genes even into non-dividing cells such as neurons and resting blood cells. The kit of choice for transfection of human preadipocytes in the 4D-Nucleofector™ X unit is the P1 primary cell kit, used in conjunction with cell type-specific protocols. The same conditions apply to the 96-well Shuttle™ or 384-well Nucleofector™ system as it is transferable across all platforms.

■Advantages

・Gene transfer efficiency: up to 90% ・Survival rate: up to 80% ・Cells can be differentiated into adipocytes after Nucleofection™

■Applications

・Confirmed in visceral and subcutaneous Poietics™ human preadipocytes ・Also tested in preadipocytes of type II diabetes. ・Previously obtained cell line results can be easily confirmed using primary cells

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    Gene introduction for primary adipocytes

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1 Models of Gene introduction for primary adipocytes

Image Part Number Price (excluding tax)
Gene introduction for primary adipocytes-Part Number-Nucleofector™ kit for human preadipocytes

Nucleofector™ kit for human preadipocytes

Available upon quote

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Lonza Co., Ltd.

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