Disulfide Bond PLUS Expression Kit CFS-EDX-DB
Cellfree Science Co., Ltd.
About This Product
■Disulfide Bond PLUS Expression Kit
-A starter kit designed to be easy to use even for first-time users.
・You can easily try synthesizing proteins containing disulfide (S-S) bonds using the wheat germ cell-free protein synthesis system.
*This product cannot be used when synthesizing and purifying a GST-tagged fusion protein, as the disulfide bonds will be cleaved by the reduced glutathione contained in the eluate during purification.
■Summary
・By applying our proprietary protein synthesis method, the "multilayer method," we have achieved the coexistence of two contradictory environments, oxidative and reductive, and created an environment in which the formed disulfide bonds can be maintained.
・Furthermore, by using human-derived disulfide bond isomerase (PDI) and oxidase Ero1α, which are required for disulfide bond formation, disulfide bonds are not formed properly, making it impossible to form and maintain higher-order structures, resulting in insolubilization. Promotes active disulfide bond formation for proteins whose activity cannot be confirmed.
■Features
- Premix type, no reagent preparation required.
・Protein synthesis is possible with just a few pipette operations.
・Target protein can be confirmed by SDS-PAGE/CBB staining.
■Recommended for the following people
・For those who would like to easily try confirming the expression of a target protein with disulfide (S-S) bonds using a wheat germ cell-free protein synthesis system.
・For those who are having trouble expressing their protein using other protein expression systems.
・For those who are having trouble with the target protein being insolubilized.
・Those who express protein but cannot confirm activity
Reagents necessary for one reaction are dispensed per tube, so no reagent adjustment is necessary. You can cut out the tubes for the number of reactions you need and use them as is.
Domestic laws and regulations
■Not applicable
・Act on Securing Biological Diversity by Regulation of the Use of Genetically Modified Organisms (Cartagena Law)
・Chemical Substance Emission Understanding and Management Promotion Act (PRTR Act)
・Chemical Substances Examination and Regulation Law (Chemical Substances Control Law)
・Poisonous and Deleterious Substances Control Law (Poisonous and Deleterious Substances Control Law)
・Industrial Safety and Health Act (Safety and Health Act)
■Precautions for use
This product is a research reagent. It cannot be used for diagnosis or treatment.
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Product
Disulfide Bond PLUS Expression Kit CFS-EDX-DB
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Handling Company
Cellfree Science Co., Ltd.
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Categories
1 Models of Disulfide Bond PLUS Expression Kit CFS-EDX-DB
| Product Image | Part Number | Price (excluding tax) | Size | Storage temperature |
|---|---|---|---|---|
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CFS-EDX-DB |
Available upon quote | 230μl x 8 reactions | -80℃ |
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