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Cell fractionation/protein extraction EzSubcell Fraction WSE-7422-WSE-7422
Cell fractionation/protein extraction EzSubcell Fraction WSE-7422-Ato

Cell fractionation/protein extraction EzSubcell Fraction WSE-7422
Ato


About This Product

A reagent kit for fractionating the nucleus, mitochondria, and cytoplasm from animal cells. This kit allows you to easily isolate organelle fractions from mammalian cells with good reproducibility and purity. A protease inhibitor and RIPA buffer are included to facilitate protein extraction after fractionation.

■Can be used for various research

・Search for drug discovery targets ・Protein localization and transport ・Signal pathway analysis ・Stress response experiment ・Epigenetics analysis, etc.

■We fractionate "nuclei", "mitochondria", and "cytoplasm" from animal cells for various experiments.

・Electrophoresis (SDS-PAGE, Native-PAGE, etc.) ・Western blotting ・Immunoprecipitation ・ELISA ・Enzyme activity experiment ・Chromatin immunoprecipitation, etc.

■Product overview

・Easy adjustment of organelle fractions with good reproducibility and purity ・The only equipment required is a refrigerated centrifuge for microtubes (~×10,000g) ・The fractionation operation for all fractions can be completed within about 2 hours. ・Use or non-use of surfactant can be selected

■Kit contents

①Fraction buffer 1: 50mL ②Fraction buffer 2: 50mL ③RIPA Lysis buffer: 20mL ④Detergent mix (50×): 1mL ⑤Protease inhibitor (100×): 0.7mL

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    Cell fractionation/protein extraction EzSubcell Fraction WSE-7422

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1 Models of Cell fractionation/protein extraction EzSubcell Fraction WSE-7422

Image Part Number Price (excluding tax) Transport temperature Capacity Shelf life How to save Sample capacity
Cell fractionation/protein extraction EzSubcell Fraction WSE-7422-Part Number-WSE-7422

WSE-7422

Available upon quote

Frozen (sometimes it is shipped refrigerated, but there is no problem with quality)

50 samples

1 year

①~③Fraction buffer 1~2, RIPA Lysis buffer: Refrigerated (4℃)
④⑤ Detergent mix, protease inhibitor: -20℃

~2.0 x 107 cells per sample

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