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This is the test method of the detergent and soap fair trading council. This is a method of measuring the number of raw bacteria in the test piece and examining the eradication effect after contacting the bacterium and the sample through the test piece. The features are as follows. ① Through a test piece. Contacts the bacteria and the sample via a sponge. There is also a sterilization test of liquid drugs as a method from passing through the test piece, which is the most basic method. (2) Add nutrients to the testing solution. Nutrient medium is added to the bacterial solution as a model dirt substance. Dirt tends to be less effective for bacteria than dirt. * The Japanese Pharmacy "Rigid surface Career Law" is a method of inoculation of bacterial solution that has not been nourished to about 5cm x 5cm (not specified in the material) and measuring the number of raw bacteria in the test piece. If you would like this, please contact us. ■ Examination method Repeat three times for test samples and contrast samples. ① Preparation of testing solution Use refined water, neutrient medium (model dirt, end concentration 1/5 times), and hard water (end concentration 3 ° DH) to be 10^7-10^8 cfu/ml. It is also possible to change the nutritional conditions for the test purpose. ② Preparation of samples Prepare as follows. ・ Test sample: The undiluted solution of the sample (liquid) or the specification (liquid / powder) of the specified concentration (the specified specification concentration of the sample or the concentration of disinfecting allegations) is prepared by the solvent (hard water, etc.). ・ Contrast sample: 0.05% (W/V) polysolbate 80 aqueous solution ③ Preparation for the test piece Punch the sponge (JISK6401 soft urethane foam, 30mm thick, no antibacterial processing) with a punch and prepare a cylindrical pillar with a diameter of 24 mm. ④ Inoculation of testing of the test solution and sample The testing solution is inoculated into the test piece, soaked it uniformly, then inoculated the sample, and soaks it uniformly. ⑤ Culture Culture at 25 ± 1 ° C for 18 hours. ⑥ Measurement of raw bacteria It is washed out of the test piece to measure the number of raw bacteria. ⑦ Evaluation Compare the number of raw bacteria in the case of test samples and contrast samples by comparing the number of raw bacteria by regular logarithm, and calculate the disinfecting activity. ■ Test bacterial species ・ STAPHYLOCCUSAUREUSNBRC12732 (Staphylococcus aureus) ・ ESCHERICHIACOLINBRC3972 (Escherichia coli) * Depending on the purpose of the test, it is possible to change the test bacterium from the specification. ■ The required amount of the sample 10ml or more * There is no problem with the large amount of sample. You can send a lot of one product, such as one product. ■ Information necessary for the test implementation It is smooth if you can get the following information when quoting. ・ Number of specimens ・ The preparation of the test sample, the solvent (in the provisions, the undiluted solution is to be used) ・ Requested effects * (except for the Japanese Report) ・ Test bacterium species (if you want to do it with bacterial species different from the rules) * If there is no request, only the Japanese report (test method and test result (determination result of the testing conditions, the number of raw bacteria after culture, the disinfecting activity value), no image). ■ Delivery date 3 weeks ~ ※Please contact us for more information. ■ Expenses Please contact us.
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Synthetic detergent for kitchen and soap for sponge, bactericidal activity test methodHandling Company
Sanitation Microbial Research Center| Image | Price (excluding tax) | Investigate content | Main sample properties |
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Available upon quote | Escillance effect by contact between samples and bacteria (via a carrier) | liquid |
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