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This is the test method of the detergent and soap fair trading council. This is a method of measuring the number of raw bacteria in the test piece and examining the eradication effect after contacting the bacterium and the sample through the test piece. The features are as follows. ① Through a test piece. The bacteria and the specimen are contacted through a circular stainless steel piece. There is also a sterilization test of liquid drugs as a method from passing through the test piece, which is the most basic method. (2) Add nutrients to the testing solution. The bacterial solution is added as a model dirt substance. Dirt tends to be less effective for bacteria than dirt. * The Japanese Pharmacy "Rigid surface Career Law" is a method of inoculation of bacterial solution that has not been nourished to about 5cm x 5cm (not specified in the material) and measuring the number of raw bacteria in the test piece. If you would like this, please contact us. ■ Examination method Repeat three times for test samples and contrast samples. ① Preparation of testing solution 1/2 Using the neutrient medium, the bacterial solution prepared to 10^8 cfu/ml and the mixture of the 30 g/L cowbumbine albumin aqueous solution are mixed. It is also possible to change the nutritional conditions for the test purpose. ② Preparation of samples Prepare as follows. ・ Test sample: The stock solution of the sample (liquid) or the specification (liquid / powder) of the sample (liquid / powder) is prepared to the specified concentration (assumed specimen concentration, or the concentration of disinfecting allegations) with a solvent (hard water). ・ Contrast sample: 0.05% (W/V) polysolbate 80 aqueous solution ③ Inoculation of testing of the test solution and samples to the test piece, static After vaccinating the test fungus solution in the stainless steel test one diameter (20 mm), the sample is vaccinated and quiet for 5 minutes. Although it is 5 minutes in the rules, it can be changed in consideration of the time expected of the effect. ④ Measurement of raw bacteria The test carrier is identified and the number of bacteria is measured. ⑤ Evaluation Compare the number of raw bacteria in the case of test samples and contrast samples by comparing the number of raw bacteria by regular logarithm, and calculate the disinfecting activity. ■ Test bacterial species ・ STAPHYLOCCUSAUREUSNBRC12732 (Staphylococcus aureus) ・ ESCHERICHIACOLINBRC3972 (Escherichia coli) * Depending on the purpose of the test, it is possible to change the test bacterium from the specification. ■ The required amount of the sample Please provide the required amount to prepare 10 ml of test samples. Also, specify the concentration and solvent of the test sample when applying for the test or sending a sample. In addition, there is no problem with the large amount of sample, so you can send a lot of one product. ■ Information necessary for the test implementation It is smooth if you can get the following information when quoting. ・ Number of specimens ・ Examination sample preparation concentration, solvent ・ Requested effects * (except for the Japanese Report) ・ Test bacterium species (if you want to do it with bacterial species different from the rules) * If there is no request, only the Japanese report (test method and test result (determination result of the testing conditions, the number of raw bacteria after culture, the disinfecting activity value), no image). ■ Delivery date 3 weeks ~ ※Please contact us for more information. ■ Expenses Please contact us.
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Synthetic detergent and soap removal activity test method for residential detergentsHandling Company
Sanitation Microbial Research Center| Image | Price (excluding tax) | Investigate content | Main sample properties |
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Available upon quote | Escillance effect by contact between samples and bacteria (via a carrier) | liquid |
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