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Nucleofector™ kits and corresponding optimization protocols are available on a variety of Nucleofector™ platforms for optimal gene transfer into human keratinocytes. The kit of choice for transfecting human keratinocytes with the 4D-Nucleofector™, 96-well Shuttle™ and 384-well Nucleofector™ systems is the P3 Primary Cell Kit. Use in conjunction with cell-specific protocols. The Nucleofector™II/2b device uses a human keratinocyte-specific kit. ■Advantages ・Gene transfer efficiency: Maximum 53% ・Survival rate: up to 60% ・Maintain cell functions such as activation (e.g., no terminal differentiation) ■Applications ・Confirmed in Clonetics™ human keratinocytes Kit is suitable for adult and neonatal keratinocytes ・Ideal for research on gene expression or intracellular signal transduction ・DNA and siRNA gene transfer cited in papers
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Nucleofector™ Kit for Human Keratinocytes (NHEK)Handling Company
Lonza Co., Ltd.| Image | Price (excluding tax) |
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Available upon quote |
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