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Nucleofector™ kits and corresponding optimization protocols are available on a variety of Nucleofector™ platforms for optimal gene transfer into human B cells. The kit of choice for transfecting human B cells in the 4D-Nucleofector™, 96-well Shuttle™ and 384-well Nucleofector™ systems is the P3 Primary Cell Kit. Use in conjunction with cell-specific protocols. The Nucleofector™II/2b device uses a human B cell specific kit. ■Advantages ・Gene transfer efficiency: Maximum 36% ・Survival rate: up to 92% ■Applications ・Ideal for human CD19+ B cells derived from peripheral blood ・Can also be applied to gene transfer of patient-derived CLL cells ・Can be used for both activated and unactivated B cells ・Sharing the same conditions for gene transfer of different substrates such as DNA, siRNA, and RNA


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    Nucleofector™ kit for human B cells

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Gene transfer for primary blood cells Nucleofector™ kit for human B cells's performance table

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