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This section provides an overview for blotting machine as well as their applications and principles. Also, please take a look at the list of 7 blotting machine manufacturers and their company rankings. Here are the top-ranked blotting machine companies as of December, 2024: 1.Precision Biosystems, 2.Cytiva, 3.Bio-Rad Laboratories, Inc..
Table of Contents
Blotting machine is a device used for transferring biological molecules (such as DNA, RNA, and proteins) from a gel after electrophoresis.
They are primarily utilized in the fields of biology and molecular biology research. Electrophoresis is an analytical technique mainly employed for the separation of proteins or DNA based on their movement properties under an applied voltage.
Firstly, in Western blotting, the separation and identification of proteins are possible. After electrophoresis, proteins are transferred onto a membrane, labeled with specific antibodies, and detected.
Blotting machines are mainly used in biological and biochemical experiments, but their applications vary depending on their types.
Western blotting is a technique for transferring and detecting proteins separated by electrophoresis on a membrane using antibodies.
The procedure involves obtaining antibodies specific to the target protein, separating the proteins through electrophoresis, transferring the obtained bands to a membrane, and performing blocking and antibody reactions to detect the target protein.
Specifically, the protein adhered to the membrane is transferred from the gel by applying voltage, reacts with antibodies specific to the target protein (primary antibody), followed by a reaction with labeled enzymes as secondary antibodies. Finally, the target band is visualized using chemiluminescence or colorimetric methods.
Southern blotting is a technique used to detect specific DNA sequences, primarily employed in molecular biology experiments. DNA samples are electrophoresed on an agarose gel, transferred (blotted) onto a membrane, and then detected using labeled probes specific to the target DNA sequence.
Probes are typically labeled with radioactive isotopes or color-producing enzymes, visualizing DNA with specific base sequences. This method is used for genome mapping, individual identification, and paternity testing.
Northern blotting is a technique for detecting specific RNA sequences, primarily used to investigate gene expression patterns and functions. It allows the study of how DNA is transcribed into mRNA and how it functions.
The procedure involves extracting DNA and RNA from cells, using DNase to isolate RNA, electrophoresing RNA on an agarose gel, transferring separated RNA to a membrane, binding complementary probes to the RNA sequence of interest, and detecting the amount and size of the target RNA.
Analyzing the movement of RNA helps in understanding the reasons for different expression patterns in organisms and the control mechanisms based on growth stages.
The principles of blotting machines involve electrophoresis, transfer, blocking and antibody reaction, detection, and results analysis.
Samples containing biomolecules are moved in a gel under an electric field, separating them by size. Gels with varying compositions are used for DNA, RNA, and proteins.
Molecules separated by electrophoresis are transferred to a membrane, replicating the separation pattern from the gel. The membrane and gel are alternately layered between filter paper and placed between two electrodes. When a voltage is applied, proteins move from the gel to the membrane following the electric current.
Blocking prevents nonspecific binding on the membrane's surface during antibody reactions, reducing false detections. Antibody reactions involve introducing antibodies that specifically bind to the target biomolecule, visualizing the target molecule through labeled substances. These combinations enable the sensitive detection of biomolecules.
Detection involves labeled substances such as antibodies or probes binding to radioisotopes, resulting in the target molecule appearing as bright bands. Luminescence or color reactions occur, and the image obtained by detection devices or cameras is processed by analysis software to evaluate the presence, quantity, size, etc., of molecules.
Results analysis processes the obtained images or data to evaluate biomolecules. It includes measuring the position and intensity of bands, background correction, creating standard curves, statistical analysis, biological interpretation, etc. This detailed analysis allows a comprehensive understanding of the presence, quantity, size, etc., of the target molecules.
When selecting a blotting device, consider the following points:
Select a device that meets the specific requirements for DNA, RNA, or protein analysis.
Check the capacity for processing the number and size of samples based on the scale and target of your research. Larger DNA fragments or high-molecular-weight proteins may require devices capable of handling corresponding gel sizes.
Ensure that the device's resolution and sensitivity meet the experiment's requirements. High sensitivity is crucial for detecting low concentrations of target molecules. Resolution is influenced by gel concentration, transfer method, and detection technique, while sensitivity depends on the detection method, labeling substances used, and device design.
Select a transfer method (semi-dry, turbo-blot, wet-blot, etc.) based on the efficient and uniform transfer of samples from the gel to the membrane. This choice is vital for the success of the blotting process.
*Including some distributors, etc.
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Precision Biosystems manufactures BlotCycler, BlotCycler-touch and BlotCycler-mini. BlotCycler is a compact bench-top device that automates western blot processing and allows clean and specific western blot assays to detect bacterial and viral disease specific proteins and antibodies. Use BlotCycler-Mini to precisely perform every automatically. Eliminate variations occurring from manual errors. Fits into new or existing workflow uses standard protocols and reagents. Any researcher interested in getting reproducible western blot assay results has to stop processing blots manually.
Bio-Rad Laboratories, Inc., founded in 1952, is an American manufacturer headquartered in Hercules, California, specialized in technological products for the life science research and clinical diagnostics markets. The company's portfolio includes life science products such as chromatography, polymerase chain reaction (PCR), and flow cytometry antibodies. In the clinical diagnostics domain, the company provides liquid chromatography (HPLC) solutions for diabetes testing, along with automation tools for hospitals and clinical core laboratories. The company also offers quality control products for laboratory quality management system as well as food and beverage testing. Notably, services offered include laboratory setup, instrument maintenance, and educational programs.
IBI Scientific was founded in 2004 and is based in Dubuque, Iowa, USA, as a manufacturer of life science research instruments and reagents. The company's product line includes the Belly Button Orbital Shaker, Hi-Lo Rocker, Ammonium Persulfate, Ampicillin Sodium Salt, and Angled BETA-GARD Shield, among others. These instruments find usage in biofilm development, electrophoresis, suppression of cell wall production, inhibition of bacteria in cell culture, and western blot treatments. The company caters to various industries including laboratory, chemical, and research.
Ranking as of December 2024
Derivation Method| Rank | Company | Click Share |
|---|---|---|
| 1 | Precision Biosystems |
66.7%
|
| 2 | Cytiva |
16.7%
|
| 3 | Bio-Rad Laboratories, Inc. |
16.7%
|
Derivation Method
The ranking is calculated based on the click share within the blotting machine page as of December 2024. Click share is defined as the total number of clicks for all companies during the period divided by the number of clicks for each company.Number of Employees
Newly Established Company
Company with a History
*Including some distributors, etc.
*Including some distributors, etc.
| Country | Number of Companies | Share (%) |
|---|---|---|
|
United States of America
|
2 | 100.0% |
